Application
Immunohistochemical analysis of paraffin-embedded H. breast section using TBX1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.Application
Western blot analysis of lysate from HLVEC cell line, using TBX1 Antibody at 1:1000 at each lane.Application
Western blot analysis in HLVEC cell line lysates (35ug/lane).Application
Fluorescent confocal image of A2058 cell stained with TBX1 Antibody . A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhalloidApplication
Formalin-fixed and paraffin-embedded human testis tissue reacted with TBX1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | TBX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | T-box transcription factor TBX1, T-box protein 1, Testis-specific T-box protein, TBX1 |
| Product description | TBX1 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.1) Fernando, R.I., et al. J. Clin. Invest. 120(2):533-544(2010) |
| Immunogen | This TBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-356 amino acids from the C-terminal region of human TBX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TBX1 |
|---|---|
| Alternative Names | T-box transcription factor TBX1 T-box protein 1 Testis-specific T-box protein TBX1 |
| Molecular Weight(MW) | 43 kDa |
Application
Immunohistochemical analysis of paraffin-embedded H. breast section using TBX1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Application
Western blot analysis of lysate from HLVEC cell line, using TBX1 Antibody at 1:1000 at each lane.
Application
Western blot analysis in HLVEC cell line lysates (35ug/lane).
Application
Fluorescent confocal image of A2058 cell stained with TBX1 Antibody . A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloid
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with TBX1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
