Application
Western blot analysis of TCCR expression in human spleen tissue lysates with TCCR antibody at 1 μg /ml.Application
Immunofluorescence of TCCR in A549 cells with TCCR antibody at 20 μg/mL.Application
Immunohistochemistry of TCCR in human spleen tissue with TCCR antibody at 10 μg/mL.| Product Name | TCCR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCCR Antibody: CRL1, TCCR, WSX1, IL27R, IL-27RA, zcytor1, CRL1, UNQ296/PRO336, Interleukin-27 receptor subunit alpha, Cytokine receptor WSX-1, IL-27 receptor subunit alpha |
| Product description | TCCR Antibody: Upon antigen challenge, T-helper cells differentiate into two functional distinct subsets, Th1 and Th2. Th1 cells produce IL-2, IFN-gamma and lymphotoxin-beta that augment cell mediated immune response while Th2 cells secrete IL-4, IL-5, and IL-10 that enhance humoral immunity. The function of T-helper cells is regulated by cytokines. A novel cytokine receptor was recently identified and cloned. It is a new member in the type I cytokine receptor family and designated TCCR for T-cell cytokine receptor and WSX-1. TCCR deficient mice had impaired Th1 responses to protein antigen challenge, including decreased levels of IFN-gamma and Th1-dependent antibody IgG2a. TCCR is predominately expressed in thymus, spleen, lymph notes and peripheral blood leukocytes.1) Chen Q, Ghilardi N, Wang H, Baker T, Xie MH, Gurney A, Grewal IS and de Sauvage FJ. Development of Th1-type immune responses requires the type I cytokine receptor TCCR Nature 2000;407(6806):916-920 |
| Immunogen | TCCR antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human TCCR precursor. The immunogen is located within the first 50 amino acids of TCCR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TCCR antibody can be used for detection of TCCR by Western blot at 1 μg/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL27RA |
|---|---|
| Alternative Names | TCCR Antibody: CRL1 TCCR WSX1 IL27R IL-27RA zcytor1 CRL1 UNQ296/PRO336 Interleukin-27 receptor subunit alpha Cytokine receptor WSX-1 IL-27 receptor subunit alpha |
| Molecular Weight(MW) | 70 kDa |
| Sequence Similarities | 3) Cat. No. 17-003 - A549 Cell Slide |
Application
Western blot analysis of TCCR expression in human spleen tissue lysates with TCCR antibody at 1 μg /ml.
Application
Immunofluorescence of TCCR in A549 cells with TCCR antibody at 20 μg/mL.
Application
Immunohistochemistry of TCCR in human spleen tissue with TCCR antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | TCCR antibody can be used for detection of TCCR by Western blot at 1 μg/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TCCR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TCCR Antibody is supplied in PBS containing 0.02% sodium azide. |
