Application
Antibody used in WB on Human Raji cells at 0.2-1 ug/ml.| Product Name | TCEB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCEB1, SIII, eloC |
| Product description | TCEB1 is known as protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Western blots using two different antibodies (P100962-T200 and P100963_P050 / P100963_P100) against two unique regions of this protein target confirm the same apparent molecular weight in our tests.1) Yu, X., et al., (2003) Science 302 (5647), 1056-1060. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCEB1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TCEB1 antibody can be used for detection of TCEB1 by ELISA at 1:7812500. TCEB1 antibody can be used for detection of TCEB1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCEB1 |
|---|---|
| Alternative Names | TCEB1 SIII eloC |
| Molecular Weight(MW) | 12 kDa |
Application
Antibody used in WB on Human Raji cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | TCEB1 antibody can be used for detection of TCEB1 by ELISA at 1:7812500. TCEB1 antibody can be used for detection of TCEB1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TCEB1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
