Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | TCF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCF2, FJHN, HNF2, LFB3, TCF2, HPC11, LF-B3, MODY5, TCF-2, VHNF1, HNF-1B, HNF1beta |
| Product description | Hepatocyte Nuclear Factor 1-. (HNF-1., Homeoprotein LFB3, Transcription factor 2, TCF2, Variant hepatic nuclear factor ) is a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The TCF2 protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1; depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes. Mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5 (Maturity-Onset of Diabetes, Type 5). A third human transcript variant is believed to exist based on such a variant in the rat: however, to date such an mRNA species has not been isolated.1) Wu, G., et al., (2004) J.Biochem.271(18), 3715-3728. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TCF2 antibody can be used for detection of TCF2 by ELISA at 1:62500. TCF2 antibody can be used for detection of TCF2 by western blot at 0.1-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNF1B |
|---|---|
| Alternative Names | TCF2 FJHN HNF2 LFB3 TCF2 HPC11 LF-B3 MODY5 TCF-2 VHNF1 HNF-1B HNF1beta |
| Molecular Weight(MW) | 45 kDa, 61 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TCF2 antibody can be used for detection of TCF2 by ELISA at 1:62500. TCF2 antibody can be used for detection of TCF2 by western blot at 0.1-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TCF2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
