Application
Western blot analysis of TCIRG1 in EL4 cell lysate with TCIRG1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | TCIRG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCIRG1 Antibody: a3, Stv1, Vph1, Atp6i, OC116, OPTB1, TIRC7, ATP6N1C, ATP6V0A3, OC-116kDa, V-type proton ATPase 116 kDa subunit a isoform 3, Osteoclastic proton pump 116 kDa subunit, V-ATPase 116 kDa isoform a3 |
| Product description | TCIRG1 Antibody: The T-cell immune regulator 1 (TCIRG1) is the a3 subunit of the vacuolar-type proton transporting ATPase (V-ATPase), a proton pump in late endosomes and lysosomes that functions in the luminal acidification of these organelles. Genetic defects in the gene encoding this protein a responsible for a severe form of autosomal recessive osteopetrosis, a condition that leads to increased bone density, decreased bone strength, and inflammation in bone tissues. TCIRG1 is also required for the normal secretion of insulin and the bacteria-killing function of macrophages.1) Toyomura T, Oka T, Yamaguchi C, et al. Three subunit a isoforms of mouse vacuolar H+-ATPase. Preferential expression of the a3 isoform during osteoclast differentiation. J. Biol. Chem. 2000; 275:8760-5 |
| Immunogen | TCIRG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCIRG1. The immunogen is located within amino acids 70 - 120 of TCIRG1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TCIRG1 antibody can be used for detection of TCIRG1 by Western blot at 0.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCIRG1 |
|---|---|
| Alternative Names | TCIRG1 Antibody: a3 Stv1 Vph1 Atp6i OC116 OPTB1 TIRC7 ATP6N1C ATP6V0A3 OC-116kDa V-type proton ATPase 116 kDa subunit a isoform 3 Osteoclastic proton pump 116 kDa subunit V-ATPase 116 kDa isoform a3 |
| Molecular Weight(MW) | 91 kDa |
| Tissue Specificity | At least two alternatively spliced isoforms are known to exist; this antibody will only detect the larger isoform. |
Application
Western blot analysis of TCIRG1 in EL4 cell lysate with TCIRG1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | TCIRG1 antibody can be used for detection of TCIRG1 by Western blot at 0.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TCIRG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TCIRG1 Antibody is supplied in PBS containing 0.02% sodium azide. |
