Application
Western blot analysis of TCTEX1D1 in K562 cell lysate with TCTEX1D1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TCTEX1D1 in K562 cells with TCTEX1D1 antibody at 20 μg/mL.| Product Name | TCTEX1D1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCTEX1D1 Antibody: Tctex1 domain-containing protein 1 |
| Product description | TCTEX1D1 Antibody: Dyneins are multi-subunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein is an approximately 12 subunit complex of two heavy chains, two intermediate chains to anchor Dynein to its cargo, four smaller intermediate chains and several light chains. It performs functions necessary for cell survival such as organelle transport and centrosome assembly. TCTEX1 is a cytoplasmic dynein light chain found in a complex with Na+ CP type X (SCN10A). TCTEX1D1, also called TCTEX1 domain containing 1, belongs to the dynein light chain TCTEX-type family. Its function is still under investigation.1) He Y, Francis F, Myers KA, et al. Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments. J. Cell Biol. 2005; 168:697-703. |
| Immunogen | TCTEX1D1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human. The immunogen is located within amino acids 30 - 80 of TCTEX1D1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| TCTEX1D1 antibody can be used for detection of TCTEX1D1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCTEX1D1 |
|---|---|
| Alternative Names | TCTEX1D1 Antibody: Tctex1 domain-containing protein 1 |
| Molecular Weight(MW) | 20 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | At least three isoforms are known to exist; this antibody will detect the two largest isoforms. TCTEX1D1 is predicted to not cross-react with other members of the TCTEX-type family. |
Application
Western blot analysis of TCTEX1D1 in K562 cell lysate with TCTEX1D1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TCTEX1D1 in K562 cells with TCTEX1D1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | TCTEX1D1 antibody can be used for detection of TCTEX1D1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TCTEX1D1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TCTEX1D1 Antibody is supplied in PBS containing 0.02% sodium azide. |
