Application
Western blot analysis of TCTN2 in SK-N-SH cell lysate with TCTN2 antibody at 1 μg/mL.| Product Name | TCTN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCTN2 Antibody: MKS8, TECT2, C12orf38, Tectonic-2 |
| Product description | TCTN2 Antibody: TCTN2 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN2 is not known, it is 49% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.1) Reiter JF and Skarnes WC. Tectonic, a novel regulator of the Hedgehog pathwy required for both activation and inhibition. Genes Dev.2006; 20:22-7. |
| Immunogen | TCTN2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TCTN2. The immunogen is located within amino acids 610 - 660 of TCTN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| TCTN2 antibody can be used for detection of TCTN2 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCTN2 |
|---|---|
| Alternative Names | TCTN2 Antibody: MKS8 TECT2 C12orf38 Tectonic-2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (75%) |
Application
Western blot analysis of TCTN2 in SK-N-SH cell lysate with TCTN2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | TCTN2 antibody can be used for detection of TCTN2 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TCTN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TCTN2 Antibody is supplied in PBS containing 0.02% sodium azide. |
