Application
Western blot analysis of TCTN3 in HeLa cell lysate with TCTN3 antibody at (A) 1 and (B) 2μg/mL.Application
Immunofluorescence of TCTN3 in HeLa cells with TCTN3 antibody at 20 μg/mL.Application
Immunocytochemistry of TCTN3 in HeLa cells with TCTN3 antibody at 4 μg/mL.| Product Name | TCTN3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCTN3 Antibody: OFD4, TECT3, JBTS18, C10orf61, PSEC0041, UNQ1881/PRO4324, Tectonic-3 |
| Product description | TCTN3 Antibody: TCTN3 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN3 is not known, it is 58% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.1) Reiter JF and Skarnes WC. Tectonic, a novel regulator of the Hedgehog pathwy required for both activation and inhibition. Genes Dev.2006; 20:22-7. |
| Immunogen | TCTN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TCTN3. The immunogen is located within amino acids 540 - 590 of TCTN3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TCTN3 antibody can be used for detection of TCTN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCTN3 |
|---|---|
| Alternative Names | TCTN3 Antibody: OFD4 TECT3 JBTS18 C10orf61 PSEC0041 UNQ1881/PRO4324 Tectonic-3 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Western blot analysis of TCTN3 in HeLa cell lysate with TCTN3 antibody at (A) 1 and (B) 2μg/mL.
Application
Immunofluorescence of TCTN3 in HeLa cells with TCTN3 antibody at 20 μg/mL.
Application
Immunocytochemistry of TCTN3 in HeLa cells with TCTN3 antibody at 4 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | TCTN3 antibody can be used for detection of TCTN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TCTN3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TCTN3 Antibody is supplied in PBS containing 0.02% sodium azide. |
