Application
Western blot analysis of TDG antibody in K562 cell line lysates (35ug/lane)Application
Flow cytometric analysis of K562 cells using TDG Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TDG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase, hTDG, TDG |
| Product description | The protein TDG belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine.1) Kim,E.J., Biochem. Biophys. Res. Commun. 377 (3), 838-842 (2008) |
| Immunogen | This TDG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the C-terminal region of human TDG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TDG |
|---|---|
| Alternative Names | G/T mismatch-specific thymine DNA glycosylase Thymine-DNA glycosylase hTDG TDG |
| Molecular Weight(MW) | 46 kDa |
Application
Western blot analysis of TDG antibody in K562 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of K562 cells using TDG Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
