Application
Western blot analysis of TDGF1 in 293 cell lysate with TDGF1 antibody at 1 μg/mL.Application
Immunofluorescence of TDGF1 in 293 cells with TDGF1 antibody at 20 μg/mL.| Product Name | TDGF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TDGF1 Antibody: CR, CRGF, CRIPTO, Teratocarcinoma-derived growth factor 1, Cripto-1 growth factor |
| Product description | TDGF1 Antibody: Teratocarcinoma-derived growth factor 1 (TDGF1 or Cripto) is a member of the epidermal growth factor-cripto FRL1 cryptic protein family and is involved in the activation of several different signaling pathways during embryonic development and cellular transformation. It is first expressed in the forming mesoderm during gastrulation but later in development the expression is restricted to the truncus arteriosus of the developing heart. This suggests that TDGF1 mediates the progression of epiblastic cells that give rise to the mesoderm. TDGF1 overexpression is characteristic of human gastric and colorectal carcinomas.1) Brandt R, Normanno N, Gullick WJ, et al. Identification and biological characterization of an epidermal growth factor-related protein: Cripto-1. J. Biol. Chem. 1994; 269:17320-8. |
| Immunogen | Rabbit polyclonal TDGF1 antibody was raised against a 17 amino acid peptide near the amino terminus of human TDGF1. The immunogen is located within amino acids 40 - 90 of TDGF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| TDGF1 antibody can be used for detection of TDGF1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TDGF1 |
|---|---|
| Alternative Names | TDGF1 Antibody: CR CRGF CRIPTO Teratocarcinoma-derived growth factor 1 Cripto-1 growth factor |
| Molecular Weight(MW) | 21 kDa |
| Sequence Similarities | 2) Cat. No. 17-010 - HEK293 Cell Slide |
Application
Western blot analysis of TDGF1 in 293 cell lysate with TDGF1 antibody at 1 μg/mL.
Application
Immunofluorescence of TDGF1 in 293 cells with TDGF1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | TDGF1 antibody can be used for detection of TDGF1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TDGF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TDGF1 Antibody is supplied in PBS containing 0.02% sodium azide. |
