Application
Western blot analysis of TDP43 in HeLa cell lysate with TDP43 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of TDP43 in Hela cells with TDP43 antibody at 20 μg/mL.Application
Immunocytochemistry of TDP43 in HeLa cells with TDP43 antibody at 5 μg/mL.| Product Name | TDP43 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TDP43 Antibody: ALS10, TDP-43, TDP43, TAR DNA-binding protein 43 |
| Product description | TDP43 Antibody: TDP43 was initially identified as a novel cellular protein that bound to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3'splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.1) Ou SH, Wu F, Garcia-Martinez LF, et al. Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs. J. Virol.1995; 69:3584-96. |
| Immunogen | TDP43 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human TDP43. The immunogen is located within amino acids 60 - 110 of TDP43. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| TDP43 antibody can be used for detection of TDP43 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TARDBP |
|---|---|
| Alternative Names | TDP43 Antibody: ALS10 TDP-43 TDP43 TAR DNA-binding protein 43 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | At least two isoforms are known to exist for this protein; this TDP43 antibody will recognize both isoforms. |
Application
Western blot analysis of TDP43 in HeLa cell lysate with TDP43 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of TDP43 in Hela cells with TDP43 antibody at 20 μg/mL.
Application
Immunocytochemistry of TDP43 in HeLa cells with TDP43 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | TDP43 antibody can be used for detection of TDP43 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TDP43 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TDP43 Antibody is supplied in PBS containing 0.02% sodium azide. |
