Application
Western blot analysis in A549 cell line lysates (35ug/lane).Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).Application
TDRD7 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TDRD7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tudor domain-containing protein 7, PCTAIRE2-binding protein, Tudor repeat associator with PCTAIRE-2, Trap, TDRD7, PCTAIRE2BP |
| Product description | TDRD7, also known as Tudor domain containing protein 7, is a component of chromatoid bodies in spermatids (named processing bodies or P-bodies in somatic cells). It is found in a mRNP complex together with TDRD1, TDRD6 and DDX4. It is also found in a complex with CABLES1, PCTK2 and PIWIL1. TDRD7 has been shown to interact with histone H3 tri-methylated at K9 in vitro.1) Lim, J., et al. Cell 125(4):801-814(2006) |
| Immunogen | This TDRD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 807-835 amino acids from the C-terminal region of human TDRD7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TDRD7 |
|---|---|
| Alternative Names | Tudor domain-containing protein 7 PCTAIRE2-binding protein Tudor repeat associator with PCTAIRE-2 Trap TDRD7 PCTAIRE2BP |
| Molecular Weight(MW) | 124 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in A549 cell line lysates (35ug/lane).
Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Application
TDRD7 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
