Application
Western blot analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of TEM1 in Human Colon tissue with TEM1 antibody at 20 μg/mL.Application
Immunohistochemistry of TEM1 in human colon tissue with TEM1 antibody at 2.5 μg/mL.| Product Name | TEM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TEM1 Antibody: TEM1, CD164L1, CD248, Endosialin, Tumor endothelial marker 1 |
| Product description | TEM1 Antibody: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.1) Rettig WJ, Garin-Chesa P, Healey JH, et al. Identification of endosialin, a cell surface glycoprotein of vascular endothelial cells in human cancer. Proc. Natl. Acad. Sci. USA 1992; 89:10832-6. |
| Immunogen | TEM1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human TEM1. The immunogen is located within the last 50 amino acids of TEM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TEM1 antibody can be used for detection of TEM1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD248 |
|---|---|
| Alternative Names | TEM1 Antibody: TEM1 CD164L1 CD248 Endosialin Tumor endothelial marker 1 |
| Molecular Weight(MW) | Predicted: 48, 83 kDa Observed: 50, 88 kDa |
| Tissue Specificity | At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms. |
Application
Western blot analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of TEM1 in Human Colon tissue with TEM1 antibody at 20 μg/mL.
Application
Immunohistochemistry of TEM1 in human colon tissue with TEM1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | TEM1 antibody can be used for detection of TEM1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TEM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TEM1 Antibody is supplied in PBS containing 0.02% sodium azide. |
