Application
Western blot analysis of TEM2 in rat colon tissue lysate with TEM2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TEM2 in Human Colon cells with TEM2 antibody at 20 μg/mL.Application
Immunohistochemistry of TEM2 in human colon tissue with TEM2 antibody at 2.5 μg/mL.| Product Name | TEM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TEM2 Antibody: Rhes, TEM2, MGC:4834, GTP-binding protein Rhes, Ras homolog enriched in striatum |
| Product description | TEM2 Antibody: Rhes, also known as tumor endothelial marker 2 (TEM2), is a small GTP-binding protein that is predominantly expressed in the striatal region of the brain. This protein belongs to the RASD subfamily of Ras-related GTP-binding protein superfamily and is thought to play a role in the normal development and function of the brain as mice lacking this gene showed increased anxiety levels and motor coordination deficits. Rhes was identified as TEM2 through analysis of genes whose expression was upregulated in tumor endothelium. Tumor endothelial markers are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies.1) Vargiu P, Morte B, Manzano J, et al. Thyroid hormone regulation of rhes, a novel RAS homolog gene expressed in the striatum. Brain Res. Mol. Brain Res.2001; 94:1-8. |
| Immunogen | TEM2 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of the human TEM2. The immunogen is located within amino acids 20 - 70 of TEM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TEM2 antibody can be used for detection of TEM2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RASD2 |
|---|---|
| Alternative Names | TEM2 Antibody: Rhes TEM2 MGC:4834 GTP-binding protein Rhes Ras homolog enriched in striatum |
| Tissue Specificity | TEM2 antibody may cross-react with the closely related protein RASD1. |
Application
Western blot analysis of TEM2 in rat colon tissue lysate with TEM2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TEM2 in Human Colon cells with TEM2 antibody at 20 μg/mL.
Application
Immunohistochemistry of TEM2 in human colon tissue with TEM2 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1472 - Rat Colon Tissue Lysate |
|---|---|
| Application Notes | TEM2 antibody can be used for detection of TEM2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TEM2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TEM2 Antibody is supplied in PBS containing 0.02% sodium azide. |
