Application
Western blot analysis of TEM4 in Jurkat cell lysate with TEM4 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunohistochemistry of TEM4 in human spleen tissue with TEM4 antibody at 2.5 μg/mL.| Product Name | TEM4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TEM4 Antibody: TEM4, P164RHOGEF, p164-RhoGEF, KIAA0337, TEM4, Rho guanine nucleotide exchange factor 17, 164 kDa Rho-specific guanine-nucleotide exchange factor |
| Product description | TEM4 Antibody: Rho GTPases, which are activated by specific guanine-nucleotide exchange factors (GEFs), play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 is a Rho-specific GEF with novel structural and regulatory properties and predominant expression in the heart. It couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful targets therapeutically.1) Ruemenapp U, Freichel-Blomquist A, Wittinghofer B, et al. A mammalian Rho-specific guanine-nucleotide exchange factor (p164-RhoGEF) without a pleckstrin homology domain. Biochem. J.2002; 366:721-8. |
| Immunogen | TEM4 antibody was raised against a 18 amino acid synthetic peptide near the center of human TEM4. The immunogen is located within amino acids 910 - 960 of TEM4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TEM4 antibody can be used for detection of TEM4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARHGEF17 |
|---|---|
| Alternative Names | TEM4 Antibody: TEM4 P164RHOGEF p164-RhoGEF KIAA0337 TEM4 Rho guanine nucleotide exchange factor 17 164 kDa Rho-specific guanine-nucleotide exchange factor |
| Molecular Weight(MW) | Predicted: 220 kDa Observed: 200 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Western blot analysis of TEM4 in Jurkat cell lysate with TEM4 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunohistochemistry of TEM4 in human spleen tissue with TEM4 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | TEM4 antibody can be used for detection of TEM4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TEM4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TEM4 Antibody is supplied in PBS containing 0.02% sodium azide. |
