Application
Western blot analysis of TEM7 in human liver tissue lysate with TEM7 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of TEM7 in mouse liver tissue with TEM7 antibody at 20 μg/mL.Application
Immunohistochemistry of TEM7 in mouse liver tissue with TEM7 antibody at 2.5 μg/mL.| Product Name | TEM7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TEM7 Antibody: TEM3, TEM7, TEM3, Plexin domain-containing protein 1, Tumor endothelial marker 3 |
| Product description | TEM7 Antibody: Tumor endothelial marker 7 (TEM7) was identified through serial analysis of gene expression on endothelial cells isolated from human normal and malignant colorectal tissues. Further experiments verified TEM7 was highly expressed in the endothelium of numerous other cancer types including breast, lung and brain tumors. At least four isoforms of TEM7 are known to exist; these include intracellular, secreted, and membrane-bound forms. A homologous protein, TEM7R (also known as PLXDC2), acts as a binding partner to TEM7 and is also abundantly expressed in the endothelium of malignant colorectal cancer but is absent or rare in normal colon mucosa. High expression of TEM7 is associated with metastasis and poor survival of patients with osteogenic sarcoma.1) St. Croix B, Rago C, Velculescu V, et al. Genes expressed in human tumor endothelium. Science2000; 289:1197-202. |
| Immunogen | TEM7 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human TEM7. The immunogen is located within the last 50 amino acids of TEM7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TEM7 antibody can be used for detection of TEM7 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLXDC1 |
|---|---|
| Alternative Names | TEM7 Antibody: TEM3 TEM7 TEM3 Plexin domain-containing protein 1 Tumor endothelial marker 3 |
Application
Western blot analysis of TEM7 in human liver tissue lysate with TEM7 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of TEM7 in mouse liver tissue with TEM7 antibody at 20 μg/mL.
Application
Immunohistochemistry of TEM7 in mouse liver tissue with TEM7 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | TEM7 antibody can be used for detection of TEM7 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TEM7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TEM7 Antibody is supplied in PBS containing 0.02% sodium azide. |
