Application
Western blot analysis of TET1 in rat brain tissue lysate with TET1 antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of TET1 in human brain tissue with TET1 antibody at 20 μg/mL.Application
Immunocytochemistry of TET1 in mouse brain tissue with TET1 antibody at 5 μg/mL.Application
Immunohistochemistry of TET1 in human brain tissue with TET1 antibody at 5 μg/mL.| Product Name | TET1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TET1 Antibody: LCX, CXXC6, bA119F7.1, KIAA1676, LCX, Methylcytosine dioxygenase TET1, CXXC-type zinc finger protein 6 |
| Product description | TET1, a member of the ten-eleven-translocation (TET) family of genes, was initially discovered as a fusion partner of MLL in acute myeloid leukemias containing the t(10;11)(q22;q23) (1). It is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine (2). TET1 expression is regulated during inflammation in both THP-1 and primary dendritic cells, and is a negative transcriptional regulator of IL-1beta following and inflammatory stimulus (3). Recent evidence has shown that TET1 is part of a signaling pathway that regulates breast cancer growth and metastasis (4).1) Lorsbach RB, Moore J, Mathew S, et al. TET1, a member of a novel protein family, is fused to MLL in acute myeloid leukemia containing the t(10;11)(q22;q23). Leukemia 2003; 17:637-41. |
| Immunogen | TET1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human TET1. The immunogen is located within amino acids 2030 - 2080 of TET1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TET1 antibody can be used for detection of TET1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TET1 |
|---|---|
| Alternative Names | TET1 Antibody: LCX CXXC6 bA119F7.1 KIAA1676 LCX Methylcytosine dioxygenase TET1 CXXC-type zinc finger protein 6 |
| Molecular Weight(MW) | Predicted: 235 kDa Observed: 240 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | TET1 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with TET2 and TET3. |
Application
Western blot analysis of TET1 in rat brain tissue lysate with TET1 antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of TET1 in human brain tissue with TET1 antibody at 20 μg/mL.
Application
Immunocytochemistry of TET1 in mouse brain tissue with TET1 antibody at 5 μg/mL.
Application
Immunohistochemistry of TET1 in human brain tissue with TET1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | TET1 antibody can be used for detection of TET1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TET1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TET1 Antibody is supplied in PBS containing 0.02% sodium azide. |
