Application
Western blot analysis of TET3 in SK-N-SH cell lysate with TET3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TET3 in human brain tissue with TET3 antibody at 20 μg/mL.Application
Immunohistochemistry of TET3 in human brain tissue with TET3 antibody at 2.5 μg/ml.| Product Name | TET3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TET3 Antibody: hCG_40738, KIAA0401, Methylcytosine dioxygenase TET3 |
| Product description | TET3 Antibody: TET3, a member of the ten-eleven-translocation (TET) family of genes, is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine and is most abundantly expressed in hematopoietic cells. Unlike the related TET2 protein, mutations in TET3 have not been observed in any myeloid malignancies. TET3 has been shown to be involved in the demethylation of zygotic DNA before the first mitosis and has been suggested to be involved in the epigenetic reprogramming of the zygotic paternal DNA following natural fertilization and may also contribute to somatic cell nuclear reprogramming during animal cloning.1) Langemeijer SM, Kuiper RP, Berends M, et al. Acquired mutations in TET2 are common in myelodysplastic syndromes. Nat. Genet. 2009; 41:838-42. |
| Immunogen | TET3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TET3. The immunogen is located within amino acids 1520 - 1570 of TET3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TET3 antibody can be used for detection of TET3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TET3 |
|---|---|
| Alternative Names | TET3 Antibody: hCG_40738 KIAA0401 Methylcytosine dioxygenase TET3 |
| Molecular Weight(MW) | 183 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least three isoforms of TET3 are known to exist. TET3 antibody is predicted to not cross react with other TET proteins. |
| Entrez Gene | 200424 |
|---|---|
| Protein Accession | NP_001274420 |
Application
Western blot analysis of TET3 in SK-N-SH cell lysate with TET3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TET3 in human brain tissue with TET3 antibody at 20 μg/mL.
Application
Immunohistochemistry of TET3 in human brain tissue with TET3 antibody at 2.5 μg/ml.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | TET3 antibody can be used for detection of TET3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TET3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TET3 Antibody is supplied in PBS containing 0.02% sodium azide. |
