Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | TFAP2A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TFAP2A, AP-2, BOFS, AP2TF, TFAP2, AP-2alpha |
| Product description | TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2 alpha is the only AP-2 protein required for early morphogenesis of the lens vesicleAP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.1) Li, M., et al., (2006) Int. J. Cancer 118 (4), 802-811. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TFAP2A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TFAP2A antibody can be used for detection of TFAP2A by ELISA at 1:312500. TFAP2A antibody can be used for detection of TFAP2A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TFAP2A |
|---|---|
| Alternative Names | TFAP2A AP-2 BOFS AP2TF TFAP2 AP-2alpha |
| Molecular Weight(MW) | 48 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TFAP2A antibody can be used for detection of TFAP2A by ELISA at 1:312500. TFAP2A antibody can be used for detection of TFAP2A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TFAP2A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
