Application
Western blot analysis of TFEB in A549 cell lysate with TFEB antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 10 μg/mL.Application
Immunohistochemistry of TFEB (NT) in human lung tissue with TFEB (NT) antibody at 5 μg/mL.| Product Name | TFEB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TFEB Antibody: TCFEB, BHLHE35, ALPHATFEB, Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35 |
| Product description | TFEB Antibody: The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors. Recent studies have shown that lysosomal biogenesis is regulated by TFEB, which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1. Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes.1) Carr CS and Sharp PA. A helix-loop-helix protein related to the immunoglobulin E box-binding proteins. Mol. Cell Biol. 1990; 10:4384-8. |
| Immunogen | TFEB antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TFEB. The immunogen is located within amino acids 70 - 120 of TFEB. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200: IF:1:100~1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TFEB |
|---|---|
| Alternative Names | TFEB Antibody: TCFEB BHLHE35 ALPHATFEB Transcription factor EB Class E basic helix-loop-helix protein 35 bHLHe35 |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
Application
Western blot analysis of TFEB in A549 cell lysate with TFEB antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 10 μg/mL.
Application
Immunohistochemistry of TFEB (NT) in human lung tissue with TFEB (NT) antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200: IF:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | TFEB antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TFEB Antibody is supplied in PBS containing 0.02% sodium azide. |
