Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TFIIIC90 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.1) Joubert, B.R., et al. Genome Med(3), 17 (2010) : |
| Immunogen | This TFIIIC90 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-244 amino acids from the N-terminal region of human TFIIIC90. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GTF3C4 |
|---|---|
| Alternative Names | General transcription factor 3C polypeptide 4 TF3C-delta Transcription factor IIIC 90 kDa subunit TFIIIC 90 kDa subunit TFIIIC90 Transcription factor IIIC subunit delta GTF3C4 |
| Molecular Weight(MW) | 92 kDa |
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
