Application
Western blot analysis of TFPI in rat small intestine tissue lysate with TFPI antibody at 1 μg/mL.Application
Immunofluorescence of TFPI in rat small intestine tissue with TFPI antibody at 20 μg/mL.Application
Immunohistochemistry of TFPI in rat small intestine tissue with TFPI antibody at 5 μg/mL.| Product Name | TFPI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TFPI Antibody: EPI, TFI, LACI, TFPI1, Tissue factor pathway inhibitor, Extrinsic pathway inhibitor, TFPI |
| Product description | TFPI Antibody: Tissue factor pathway inhibitor (TFPI), also known as lipoprotein-associated coagulation inhibitor, is a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation (1). TFPI is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. TFPI inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop (1).1) Broze GJ Jr and Girard TJ. Tissue factor pathway inhibitor: structure-function. Front. Biosci. 17:262-80. |
| Immunogen | TFPI antibody was raised against a 13 amino acid peptide near the amino terminus of human TFPI. The immunogen is located within amino acids 70 - 120 of TFPI. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TFPI antibody can be used for detection of TFPI by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TFPI |
|---|---|
| Alternative Names | TFPI Antibody: EPI TFI LACI TFPI1 Tissue factor pathway inhibitor Extrinsic pathway inhibitor TFPI |
| Molecular Weight(MW) | Predicted: 33 kDa Observed: 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rabbit: (92%) |
| Tissue Specificity | TFPI antibody is human, mouse and rat reactive. At least two isoforms of TFPI are known to exist; this antibody will detect both isoforms. |
Application
Western blot analysis of TFPI in rat small intestine tissue lysate with TFPI antibody at 1 μg/mL.
Application
Immunofluorescence of TFPI in rat small intestine tissue with TFPI antibody at 20 μg/mL.
Application
Immunohistochemistry of TFPI in rat small intestine tissue with TFPI antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate |
|---|---|
| Application Notes | TFPI antibody can be used for detection of TFPI by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TFPI antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TFPI Antibody is supplied in PBS containing 0.02% sodium azide. |
