The homeobox gene superfamily is highly conserved during evolution. These members act as transcription factors during several important developmental processes through their 60 amino acid homeodomains that recognize and bind to the regulatory region of their target genes. Tgifx1, the product of a novel testis homeobox gene, may play a crucial role during spermatogenesis.1) Jin, L., et al., (2005) Gene Expr. Patterns 5 (4), 457-462.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse TGIFX1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
TGIFX1 antibody can be used for detection of TGIFX1 by ELISA at 1:312500. TGIFX1 antibody can be used for detection of TGIFX1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Testis at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1416 - Mouse Testis Tissue Lysate
Application Notes
TGIFX1 antibody can be used for detection of TGIFX1 by ELISA at 1:312500. TGIFX1 antibody can be used for detection of TGIFX1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TGIFX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.