Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | TGM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, TGM2 is the autoantigen implicated in celiac disease.Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.1) Li, Q., (2006) Endocrinology 147 (5), 2147-2154. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TGM2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TGM2 antibody can be used for detection of TGM2 by ELISA at 1:312500. TGM2 antibody can be used for detection of TGM2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TGM2 |
|---|---|
| Alternative Names | TGM2 TG2 TGC GNAH HEL-S-45 G-ALPHA-h |
| Molecular Weight(MW) | 77 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TGM2 antibody can be used for detection of TGM2 by ELISA at 1:312500. TGM2 antibody can be used for detection of TGM2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TGM2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
