Application
Western blot analysis of TGM5 in rat heart tissue lysate with TGM5 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TGM5 in mouse heart tissue with TGM5 antibody at 20 μg/mL.Application
Immunohistochemistry of TGM5 in mouse heart tissue with TGM5 antibody at 2.5 μg/mL.| Product Name | TGM5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | TGM5 Antibody: Transglutaminases (TGM) are a family of structurally and functionally related Ca2+-dependent enzymes (TGases) that stabilize protein assemblies through the formation of gamma-glutamyl-epsilon lysine crosslinks. TGases influence numerous biological processes, including blood coagulation, cell differentiation, fertilization and apoptosis. TGM5 belongs to the transglutaminase superfamily and catalyzes the cross-linking of proteins between glutamine and lysine residues, often resulting in stabilization of protein assemblies. TGM5 is also expressed in the human hair follicle. Defects in TGM5 are associated with acral peeling skin syndrome.1) Ueki S, et al. Dual functions of transglutaminase in novel cell adhesion. J. Cell Sci. 1996; 109:2727-35. |
| Immunogen | TGM5 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TGM5. The immunogen is located within the last 50 amino acids of TGM5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TGM5 antibody can be used for detection of TGM5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TGM5 |
|---|---|
| Alternative Names | TGM5 Antibody: TGX TGM6 TGMX TGASE5 TGASEX Protein-glutamine gamma-glutamyltransferase 5 Transglutaminase X TGX |
| Tissue Specificity | TGM5 antibody is predicted to not cross-react with other TGase protein family members. Multiple isoforms of TGM5 are known to exist. |
Application
Western blot analysis of TGM5 in rat heart tissue lysate with TGM5 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TGM5 in mouse heart tissue with TGM5 antibody at 20 μg/mL.
Application
Immunohistochemistry of TGM5 in mouse heart tissue with TGM5 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1461 - Rat Heart Tissue Lysate |
|---|---|
| Application Notes | TGM5 antibody can be used for detection of TGM5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | TGM5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TGM5 Antibody is supplied in PBS containing 0.02% sodium azide. |
