Application
Western blot analysis of THBS4 in mouse lung tissue lysate with THBS4 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of THBS4 in human lung cells with THBS4 antibody at 20 μg/mL.| Product Name | THBS4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | THBS4 Antibody: TSP4, TSP4, Thrombospondin-4 |
| Product description | THBS4 Antibody: Thrombospondin-4 (THBS4) belongs to the thrombospondin protein family, a family of adhesive calcium-binding glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. Studies suggest that this protein may be involved in local signaling in the developing and adult nervous system. THBS4 is widely expressed and is abundant in atherosclerotic lesions and in areas prone to the development of lesions. It has been suggested to play an important role in the recruitment of macrophages and the local regulation of inflammation associated with atherogenesis. THBS4 has also been suggested as a putative tumor-suppressor protein in colorectal cancer.1) Arber S and Caroni P. Thrombospondin-4, an extracellular matrix protein expressed in the developing and adult nervous system promotes neurite outgrowth. J. Cell Biol. 1995; 131:1083-94 |
| Immunogen | THBS4 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human THBS4. The immunogen is located within the first 50 amino acids of THBS4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| THBS4 antibody can be used for detection of THBS4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | THBS4 |
|---|---|
| Alternative Names | THBS4 Antibody: TSP4 TSP4 Thrombospondin-4 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (93%), Bovine: (80%) |
| Tissue Specificity | At least two isoforms of THBS4 are known to exist; this antibody will only detect the longer isoform. |
Application
Western blot analysis of THBS4 in mouse lung tissue lysate with THBS4 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of THBS4 in human lung cells with THBS4 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | THBS4 antibody can be used for detection of THBS4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | THBS4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | THBS4 Antibody is supplied in PBS containing 0.02% sodium azide. |
