Application
Western blot of hippocampal lysate showing specific immunolabeling of the ~50k TR-alpha1 and the ~58k TR-alpha2 protein.| Product Name | Thyroid Hormone Receptor Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AR7, EAR7, ERBA, CHNG6, ERBA1, NR1A1, THRA1, THRA2, ERB-T-1, c-ERBA-1, Nuclear receptor subfamily 1 group A member 1, EAR-7 |
| Product description | Thyroid hormones are essential for development of the central nervous system and deficits in these hormones during development affects such cognitive functions as learning and memory (Ambrogini et al., 2005; Chan and Kilby, 2000). Thyroid hormones exert their physiological role mainly through binding to specific nuclear receptors including the predominant isoforms of thyroid hormone receptors TRalpha1, TRalpha2, TRbeta1 and TRbeta2. TRalpha1, TRbeta1 and TRbeta2 bind T3 with high affinity and also bind to thyroid hormone response elements (TREs) on chromatin to regulate the transcriptional processes in several target tissues, including adult rat brain (Constantinou et al., 2005).1) Ambrogini P, Cuppini R, Ferri P, Mancini C, Ciaroni S, Voci A, Gerdoni E, Gallo G (2005) Thyroid hormones affect neurogenesis in the dentate gyrus of adult rat. Neuroendocrinology 81:244-253. |
| Immunogen | Peptide corresponding to amino acid residues from the N-terminal region of human thyroid hormone receptor, a1/a2-isotype. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with canine, mouse and rat based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
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| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | THRA |
|---|---|
| Alternative Names | AR7 EAR7 ERBA CHNG6 ERBA1 NR1A1 THRA1 THRA2 ERB-T-1 c-ERBA-1 Nuclear receptor subfamily 1 group A member 1 EAR-7 |
| Molecular Weight(MW) | 50/58 |
Application
Western blot of hippocampal lysate showing specific immunolabeling of the ~50k TR-alpha1 and the ~58k TR-alpha2 protein.| Application Notes | The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with canine, mouse and rat based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
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| Form | Liquid |
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| Storage Instructions | Thyroid Hormone Receptor antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
