Application
Western blot analysis of TIAF in K562 cell lysate with TIAF antibody at 1 μg/mL.Application
Immunocytochemistry of TIAF in K562 cells with TIAF antibody at 2.5 μg/ml.| Product Name | TIAF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TIAF Antibody: MAJN, SPR210, TGFB1-induced anti-apoptotic factor 1, 12 kDa TGF-beta-1-induced antiapoptotic factor |
| Product description | TIAF Antibody: TIAF-1 (TGFB1-induced anti-apoptotic factor 1), also known as MYO18A, is a TGFB1-induced anti-apoptotic factor that functions to inhibit the cytotoxic effects of TNF-alpha, TRADD and FADD. It localizes to the nucleus and significantly up-regulated in activated helper T lymphocytes (TH2) in chronic kidney and liver allograft rejection. Suppression of TIAF1 expression by siRNA prevents UV irradiation-mediated p53 phosphorylation and nuclear translocation. TIAF1 is essential in the p53- and WOX1-mediated cell death and shows an aggregation-dependent control of tumor progression and metastasis, and regulation of cell death.1) Chang NS, Mattison J, Cao H, et al. Cloning and characterization of a novel transforming growth factor-beta1-induced TIAF1 protein that inhibits tumor necrosis factor cytotoxicity. Biochem. Biophys. Res. Commun. 1998; 253:743-9. |
| Immunogen | TIAF antibody was raised against a 16 amino acid peptide near the amino terminus of human TIAF . The immunogen is located within the first 50 amino acids of TIAF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| TIAF Antibody can be used for detection of TIAF by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TIAF |
|---|---|
| Alternative Names | TIAF Antibody: MAJN SPR210 TGFB1-induced anti-apoptotic factor 1 12 kDa TGF-beta-1-induced antiapoptotic factor |
| Molecular Weight(MW) | Predicted: 13 kDa Observed: 14 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | Two alternatively spliced transcript variants encoding different isoforms have been identified. |
Application
Western blot analysis of TIAF in K562 cell lysate with TIAF antibody at 1 μg/mL.
Application
Immunocytochemistry of TIAF in K562 cells with TIAF antibody at 2.5 μg/ml.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | TIAF Antibody can be used for detection of TIAF by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TIAF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TIAF Antibody is supplied in PBS containing 0.02% sodium azide. |
