Application
Western blot analysis of anti-TIEG Antibody pre-incubated with and without blocking peptide in CEM cell line lysate.Application
Western Blot at 1:2000 dilution Lane 1: 293T whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: human kidney lysates Lane 4: human lung lysates Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TIEG antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | TIEG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Krueppel-like factor 10, EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1, TGFB-inducible early growth response protein 1, TIEG-1, KLF10, TIEG, TIEG1 |
| Product description | TIEG is a transcriptional repressor involved in the regulation of cell growth. TIEG binds to the consensus sequence 5'-GGTGTG-3'and inhibits cell growth.1) Hawse,J.R., Mol. Endocrinol. 22 (7), 1579-1595 (2008) |
| Immunogen | This TIEG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human TIEG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLF10 |
|---|---|
| Alternative Names | Krueppel-like factor 10 EGR-alpha Transforming growth factor-beta-inducible early growth response protein 1 TGFB-inducible early growth response protein 1 TIEG-1 KLF10 TIEG TIEG1 |
| Molecular Weight(MW) | 53 kDa |
Application
Western blot analysis of anti-TIEG Antibody pre-incubated with and without blocking peptide in CEM cell line lysate.
Application
Western Blot at 1:2000 dilution Lane 1: 293T whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: human kidney lysates Lane 4: human lung lysates Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TIEG antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
