Application
Western Blot at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | TIFA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAF-interacting protein with FHA domain-containing protein A, Putative MAPK-activating protein PM14, Putative NF-kappa-B-activating protein 20, TRAF2-binding protein, TIFA, T2BP |
| Product description | Adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. Induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism.1) Minoda, Y., et al. Biochem. Biophys. Res. Commun. 344(3):1023-1030(2006) |
| Immunogen | This TIFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-79 amino acids from the N-terminal region of human TIFA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TIFA |
|---|---|
| Alternative Names | TRAF-interacting protein with FHA domain-containing protein A Putative MAPK-activating protein PM14 Putative NF-kappa-B-activating protein 20 TRAF2-binding protein TIFA T2BP |
| Molecular Weight(MW) | 21 kDa |
Application
Western Blot at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
