Application
Western blot analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TIM-4 in Jurkat cells with TIM-4 antibody at 20 μg/mL.Application
Immunohistochemistry of TIM-4 in human spleen tissue with TIM-4 antibody at 2.5 μg/ml.Application
Immunofluorescence of TIM-4 in human spleen tissue with TIM-4 antibody at 20 μg/ml.Application
Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 μg/mL.| Product Name | TIM-4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TIM-4 Antibody: TIM4, SMUCKLER, TIM4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, TIMD-4 |
| Product description | TIM-4 Antibody: The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.1) Meyers JH, Sabatos CA, Chakravarti S, et al. The TIM family regulates autoimmune and allergic diseases. Trends Mol. Med. 2005; 11:362-9. |
| Immunogen | TIM-4 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-4. The immunogen is located within amino acids 60 - 110 of TIM-4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| TIM-4 antibody can be used for the detection of TIM-4 by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TIMD4 |
|---|---|
| Alternative Names | TIM-4 Antibody: TIM4 SMUCKLER TIM4 T-cell immunoglobulin and mucin domain-containing protein 4 T-cell immunoglobulin mucin receptor 4 TIMD-4 |
| Molecular Weight(MW) | Observed: 45 kDa |
| Sequence Similarities | 3) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | At least four isoforms of TIM-4 are known to exist; this antibody will detect all four isoforms. |
Application
Western blot analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TIM-4 in Jurkat cells with TIM-4 antibody at 20 μg/mL.
Application
Immunohistochemistry of TIM-4 in human spleen tissue with TIM-4 antibody at 2.5 μg/ml.
Application
Immunofluorescence of TIM-4 in human spleen tissue with TIM-4 antibody at 20 μg/ml.
Application
Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | TIM-4 antibody can be used for the detection of TIM-4 by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TIM-4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TIM-4 Antibody is supplied in PBS containing 0.02% sodium azide. |
