Application
Western blot analysis of TIP47 in Daudi cell lysate with TIP47 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of TIP47 in Rat Liver cells with TIP47 antibody at 20 μg/mL.Application
Immunohistochemistry of TIP47 in rat liver tissue with TIP47 antibody at 10 μg/mL.| Product Name | TIP47 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TIP47 Antibody: PP17, TIP47, M6PRBP1, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein |
| Product description | TIP47 Antibody: Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.1) Barbero P, Bittova L, and Pfeffer SR. Visualization of Rab9-mediated vesicle transport from endosomes to the trans Golgi in living cells. J. Cell Biol. 2002; 156:511-8. |
| Immunogen | TIP47 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TIP47. The immunogen is located within the last 50 amino acids of TIP47. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| TIP47 antibody can be used for the detection of TIP47 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | M6PRBP1 |
|---|---|
| Alternative Names | TIP47 Antibody: PP17 TIP47 M6PRBP1 Perilipin-3 47 kDa mannose 6-phosphate receptor-binding protein 47 kDa MPR-binding protein |
| Sequence Similarities | 2) Cat. No. 1464 - Rat Liver Tissue Lysate |
Application
Western blot analysis of TIP47 in Daudi cell lysate with TIP47 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of TIP47 in Rat Liver cells with TIP47 antibody at 20 μg/mL.
Application
Immunohistochemistry of TIP47 in rat liver tissue with TIP47 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | TIP47 antibody can be used for the detection of TIP47 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TIP47 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TIP47 Antibody is supplied in PBS containing 0.02% sodium azide. |
