Application
Western blot analysis of TIRP in human (A), mouse (B), and rat (C) kidney cell lysates with TIRP antibody (C2) at 1 μg/mL.Application
Immunofluorescence of TIRP in Mouse Kidney cells with TIRP antibody at 20 μg/mL.Application
Immunohistochemical staining of mouse kidney using TIRP antibody at 2 μg/mL.| Product Name | TIRP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TIRP Antibody: TIRP, TRAM, TIRAP3, MyD88-4, TICAM-2, TIRP, TIR domain-containing adapter molecule 2, Putative NF-kappa-B-activating protein 502 |
| Product description | TIRP Antibody: TIRP is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TIRP, along with other molecules such as TRIF, MAL, and MyD88, serves as an adaptor protein that allows for the interaction and activation of the IL-1R-associated kinase (IRAK) family, the subsequent activation of TNF receptor associated factor (TRAF)-6, and ultimately the activation of NF-κB. Expression of TIRP appears to be essential for TLR4 signalling.1) O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003;24:286-9. |
| Immunogen | TIRP antibody was raised against a peptide corresponding to amino acids near the C-terminus of human TIRP. The immunogen is located within the last 50 amino acids of TIRP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TIRP antibody can be used for detection of TIRP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TICAM2 |
|---|---|
| Alternative Names | TIRP Antibody: TIRP TRAM TIRAP3 MyD88-4 TICAM-2 TIRP TIR domain-containing adapter molecule 2 Putative NF-kappa-B-activating protein 502 |
| Sequence Similarities | 3) Cat. No. 1465 - Rat Kidney Tissue Lysate |
Application
Western blot analysis of TIRP in human (A), mouse (B), and rat (C) kidney cell lysates with TIRP antibody (C2) at 1 μg/mL.
Application
Immunofluorescence of TIRP in Mouse Kidney cells with TIRP antibody at 20 μg/mL.
Application
Immunohistochemical staining of mouse kidney using TIRP antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1324 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | TIRP antibody can be used for detection of TIRP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TIRP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TIRP Antibody is supplied in PBS containing 0.02% sodium azide. |
