Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Product Name | TLE3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLE3, ESG, ESG3, GRG3, HsT18976 |
| Product description | The TLE genes are differentially expressed and encode nuclear proteins, consistent with the presence of sequence motifs associated with nuclear functions. These genes are the human homologues of Drosophila groucho, a member of the Notch signaling pathway which controls a number of different cell-fate choices in invertebrates and vertebrates.1) Liu, Y., et al., (1996) Genomics 31 (1), 58-64. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TLE3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TLE3 antibody can be used for detection of TLE3 by ELISA at 1:62500. TLE3 antibody can be used for detection of TLE3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TLE3 |
|---|---|
| Alternative Names | TLE3 ESG ESG3 GRG3 HsT18976 |
| Molecular Weight(MW) | 83 kDa |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TLE3 antibody can be used for detection of TLE3 by ELISA at 1:62500. TLE3 antibody can be used for detection of TLE3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TLE3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
