Application
Western blot analysis of TLR10 in human lymph node cell lysates with TLR10 antibody (IN) at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of TLR10 in Daudi cells with TLR10 antibody at 2 μg/mL.| Product Name | TLR10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR10 Antibody: CD290, Toll-like receptor 10 |
| Product description | TLR10 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR10 is highly homologous to TLRs 1 and 6 and is most highly expressed in lymphoid tissues. Most recently genetic variation in TLR10 has been associated with contributing to asthma risk. It should be noted that TLR10 exists in mice only as a pseudogene.1) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. |
| Immunogen | TLR10 antibody was raised against a peptide corresponding to 14 amino acids near the middle of human TLR10. The immunogen is located within amino acids 600 - 650 of TLR10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| TLR10 antibody can be used for detection of TLR10 by Western blot at 0.5 to 1 μg/mL. Despite its predicted size of ~90 kDa, a band correpsonding to TLR10 is observed at ~75 kDa in immunoblot assays. For immunofluorescence start at 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR10 |
|---|---|
| Alternative Names | TLR10 Antibody: CD290 Toll-like receptor 10 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Western blot analysis of TLR10 in human lymph node cell lysates with TLR10 antibody (IN) at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of TLR10 in Daudi cells with TLR10 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1369 - Human Lymph node Tissue Lysate |
|---|---|
| Application Notes | TLR10 antibody can be used for detection of TLR10 by Western blot at 0.5 to 1 μg/mL. Despite its predicted size of ~90 kDa, a band correpsonding to TLR10 is observed at ~75 kDa in immunoblot assays. For immunofluorescence start at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR10 Antibody is supplied in PBS containing 0.02% sodium azide. |
