Application
Western blot analysis of TLR5 in THP-1 cell lysate with TLR5 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of TLR5 in THP1 cells with TLR5 antibody at 10 μg/mL.Application
Immunocytochemistry of TLR5 in THP-1 cells with TLR5 antibody at 10 μg/mL.| Product Name | TLR5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR5 Antibody: TIL3, SLEB1, MELIOS, TIL3, Toll-like receptor 5 |
| Product description | TLR5 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF-κB, leading to the activation of TNF-α and other cytokines. A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires'disease and demonstrates the importance of TLR5 in the innate immune response.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol.2003; 21:335-76. |
| Immunogen | TLR5 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR5. The immunogen is located within the last 50 amino acids of TLR5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| TLR5 antibody can be used for detection of TLR5 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR5 |
|---|---|
| Alternative Names | TLR5 Antibody: TIL3 SLEB1 MELIOS TIL3 Toll-like receptor 5 |
| Sequence Similarities | 2) Cat. No. 17-008 - THP-1 Cell Slide |
Application
Western blot analysis of TLR5 in THP-1 cell lysate with TLR5 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of TLR5 in THP1 cells with TLR5 antibody at 10 μg/mL.
Application
Immunocytochemistry of TLR5 in THP-1 cells with TLR5 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1208 - THP-1 Cell Lysate |
|---|---|
| Application Notes | TLR5 antibody can be used for detection of TLR5 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR5 Antibody is supplied in PBS containing 0.02% sodium azide. |
