Application
Western blot analysis of TLR6 in Daudi cell lysate with TLR6 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunocytochemistry of TLR6 in Jurkat cells with TLR6 antibody at 10 μg/mL.| Product Name | TLR6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR6 Antibody: CD286, Toll-like receptor 6 |
| Product description | TLR6 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR6 was first identified as a close homolog of TLR1, sharing 69% sequence identify. Like TLR1, TLR6 can form heterodimers with TLR2, and these TLR6:TLR2 dimers coordinate macrophage activation by Gram-positive bacteria and the yeast cell wall particle zymosan. Activation of these complexes not only initiates pro-inflammatory cascades, but also can lead to apoptotic responses.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. |
| Immunogen | TLR6 antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human TLR6. The immunogen is located within amino acids 160 - 210 of TLR6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| TLR6 antibody can be used for detection of TLR6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR6 |
|---|---|
| Alternative Names | TLR6 Antibody: CD286 Toll-like receptor 6 |
| Molecular Weight(MW) | Observed: 91 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | This TLR6 antibody is predicted to have no cross-reactivity to other members of the TLR protein family. |
Application
Western blot analysis of TLR6 in Daudi cell lysate with TLR6 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunocytochemistry of TLR6 in Jurkat cells with TLR6 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | TLR6 antibody can be used for detection of TLR6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR6 Antibody is supplied in PBS containing 0.02% sodium azide. |
