WB
All lanes: TLR6 antibody at 5µg/ml + Jurkat whole cell lysate, Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution.IHC
Immunohistochemistry of paraffin-embedded human thyroid tissue using TLR6 Antibody at dilution of 1:100.ICC/IF
Immunofluorescence staining of A549 cells with TLR6 Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-TLR6 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Toll-like receptor 6 protein (32-586AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR6 |
|---|---|
| Gene Synonyms | CD286 |
| Gene Full Name | toll like receptor 6 |
| Gene Summary | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor functionally interacts with toll-like receptor 2 to mediate cellular response to bacterial lipoproteins. A Ser249Pro polymorphism in the extracellular domain of the encoded protein may be associated with an increased of asthma is some populations.[provided by RefSeq, Jan 2011] |
| Alternative Names | CD286 antibody CD286 antigen antibody TLR 6 antibody Tlr6 antibody TLR6_HUMAN antibody Toll like receptor 6 precursor antibody Toll-like receptor 6 antibody |
| Molecular Weight(MW) | 92kDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein. Membrane raft. Golgi apparatus. |
WB
All lanes: TLR6 antibody at 5µg/ml + Jurkat whole cell lysate, Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human thyroid tissue using TLR6 Antibody at dilution of 1:100.
ICC/IF
Immunofluorescence staining of A549 cells with TLR6 Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
