Application
Western blot analysis of TLR7 in Daudi cell lysates with TLR7 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.Application
Immunocytochemistry staining of Daudi cells using TLR7 antibody at 2 μg/mL.| Product Name | TLR7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR7 Antibody: Toll-like receptor 7 |
| Product description | TLR7 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR7, like TLRs 3, 8, and 9, is localized in intracellular acidic compartments such as the phagolysosome and will recognize some single-stranded RNA viruses such as vesicular stomatitis virus (VSV) and influenza virus. Activation of TLR7 by VSV results in stimulation of the immune response including IFNalpha secretion, suggesting the importance of TLR7 in virus recognition.1) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. |
| Immunogen | TLR7 antibody was raised against a peptide corresponding to 14 amino acids near the center of mouse TLR7. The immunogen is located within amino acids 420 - 470 of TLR7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| TLR7 antibody can be used for detection of TLR7 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Tlr7 |
|---|---|
| Alternative Names | TLR7 Antibody: Toll-like receptor 7 |
| Molecular Weight(MW) | Predicted: 116 kDa Observed: 135 kDa |
Application
Western blot analysis of TLR7 in Daudi cell lysates with TLR7 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Application
Immunocytochemistry staining of Daudi cells using TLR7 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | TLR7 antibody can be used for detection of TLR7 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR7 Antibody is supplied in PBS containing 0.02% sodium azide. |
