Application
Western blot analysis in Ramos cell line lysates (35ug/lane).Application
TM55B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TM55B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase, Type 1 PtdIns-4,5-P2 4-Ptase, PtdIns-4,5-P2 4-Ptase I, Transmembrane protein 55B, TMEM55B, C14orf9 |
| Product description | TMEM55B catalyzes the degradation of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P2) by removing the 4-phosphate (Ungewickell et al., 2005 [PubMed 16365287]).1) Zou, J., et al. Proc. Natl. Acad. Sci. U.S.A. 104(43):16834-16839(2007) |
| Immunogen | This TM55B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-211 amino acids from the C-terminal region of human TM55B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM55B |
|---|---|
| Alternative Names | Type 1 phosphatidylinositol 4 5-bisphosphate 4-phosphatase Type 1 PtdIns-4 5-P2 4-Ptase PtdIns-4 5-P2 4-Ptase I Transmembrane protein 55B TMEM55B C14orf9 |
| Molecular Weight(MW) | 29 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis in Ramos cell line lysates (35ug/lane).
Application
TM55B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
