Application
Western blot analysis of TMEM16B in human small intestine tissue lysate with TMEM16B antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TMEM16B in Human Brain cells with TMEM16B antibody at 20 μg/mL.Application
Immunohistochemistry of TMEM16B in human brain tissue with FRMPD4 antibody at 5 μg/mL.| Product Name | TMEM16B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TMEM16B Antibody: C12orf3, TMEM16B, C12orf3, Anoctamin-2, Transmembrane protein 16B |
| Product description | TMEM16B Antibody: Calcium-activated chloride channels (CaCC) are present in many cell types and mediate physiological functions such as epithelial secretion, sensory signal transduction, and smooth muscle contraction. Subunits of these CaCC's include the transmembrane proteins TMEM16A and TMEM16B. TMEM16B is predicted to have eight transmembrane domains with both the amino and carboxy termini in the cytoplasm and is expressed in several tissues including olfactory sensory neurons as well as photoreceptors in mammalian retina. Like TMEM16A, TMEM16B is thought to form at least part of CaCC's but has different biophysical characteristics such as voltage dependence and unitary conductance.1) Schroeder BC, Cheng T, Jan YN, et al. Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. Cell2008; 134:1019-29. |
| Immunogen | TMEM16B antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human TMEM16B. The immunogen is located within the first 50 amino acids of TMEM16B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TMEM16B antibody can be used for detection of TMEM16B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANO2 |
|---|---|
| Alternative Names | TMEM16B Antibody: C12orf3 TMEM16B C12orf3 Anoctamin-2 Transmembrane protein 16B |
| Molecular Weight(MW) | Predicted: 110 kDa Observed: 107 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Entrez Gene | 57101 |
|---|---|
| Protein Accession | NP_001265525 |
Application
Western blot analysis of TMEM16B in human small intestine tissue lysate with TMEM16B antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TMEM16B in Human Brain cells with TMEM16B antibody at 20 μg/mL.
Application
Immunohistochemistry of TMEM16B in human brain tissue with FRMPD4 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1308 - Human Small Intestine Tissue Lysate |
|---|---|
| Application Notes | TMEM16B antibody can be used for detection of TMEM16B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TMEM16B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TMEM16B Antibody is supplied in PBS containing 0.02% sodium azide. |
