Application
Western blot analysis of TMEM18 in rat brain lysate with TMEM18 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of TMEM18 in Mouse Brain cells with TMEM18 antibody at 20 μg/mL.Application
Immunohistochemistry of TMEM18 in mouse brain tissue with TMEM18 antibody at 2.5 μg/mL.| Product Name | TMEM18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TMEM18 Antibody: Transmembrane protein 18 |
| Product description | TMEM18 Antibody: Recent studies have examined how overexpression of certain genes enhances the tropism of neural stem cells (NSCs) for gliomas. Overexpression of Transmembrane protein 18 (TMEM18) provides NSCs and neural precursors an increased migration capacity towards glioblastoma cells in vitro and in the rat brain, while functional inactivation of the gene resulted in an almost complete loss of migration activity, suggesting that TMEM18 could be a potential target in NSC-based glioma therapy. Other recent results suggest that among others, the TMEM18 loci is associated with higher than normal body mass index (BMI) and obesity, indicating there may be a CNS-related role in predisposition to obesity. Multiple isoforms of TMEM18 are known to exist.1) Jurvansuu J, Zhao Y, Leung DS, et al. Transmembrane protein 18 enhances the tropism of neural stem cells for glioma cells. Cancer Res.2008; 68:4614-22. |
| Immunogen | TMEM18 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TMEM18. The immunogen is located within the last 50 amino acids of TMEM18. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TMEM18 antibody can be used for detection of TMEM18 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM18 |
|---|---|
| Alternative Names | TMEM18 Antibody: Transmembrane protein 18 |
Application
Western blot analysis of TMEM18 in rat brain lysate with TMEM18 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of TMEM18 in Mouse Brain cells with TMEM18 antibody at 20 μg/mL.
Application
Immunohistochemistry of TMEM18 in mouse brain tissue with TMEM18 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | TMEM18 antibody can be used for detection of TMEM18 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TMEM18 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TMEM18 Antibody is supplied in PBS containing 0.02% sodium azide. |
