Application
Western blot analysis of TMEM38A in rat skeletal muscle tissue lysate with TMEM38A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TMEM38A in mouse skeletal muscle tissue with TMEM38A antibody at 20 μg/mL.| Product Name | TMEM38A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TMEM38A Antibody: TRICA, TRIC-A, Trimeric intracellular cation channel type A, Transmembrane protein 38A |
| Product description | TMEM38A Antibody: TMEM38A and TMEM38B are two recently identified trimeric intracellular cation (TRIC) channel subtypes. TMEM38A is preferentially expressed in excitable tissues such as striated muscle and brain and localizes to the sarcoplasmic reticulum (SR) in muscle tissues. Mice deficient in both TMEM38A and TMEM38B suffer embryonic cardiac failure; the cardiac myocytes display severe dysfunction in SR Ca2+ handling, weakened Ca2+ release, and reduced K+ permeability indicating that the TRIC cation channels are likely to act as counter-ion channels that function in synchronization with Ca2+ release from intracellular stores. Other experiments have shown that TMEM38A and TMEM38B can act with junctophilin proteins to support efficient ryanodine receptor-mediated Ca2+ release in muscle cells.1) Yazawa M, Ferrante C, Feng J, et al. TRIC channels are essential for Ca2+ handling in intracellular stores. Nature2007; 448:78-82. |
| Immunogen | TMEM38A antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human TMEM38A. The immunogen is located within the last 50 amino acids of TMEM38A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| TMEM38A antibody can be used for detection of TMEM38A by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM38A |
|---|---|
| Alternative Names | TMEM38A Antibody: TRICA TRIC-A Trimeric intracellular cation channel type A Transmembrane protein 38A |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rabbit: (94%), Bovine: (94%), Chicken: (76%) |
Application
Western blot analysis of TMEM38A in rat skeletal muscle tissue lysate with TMEM38A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TMEM38A in mouse skeletal muscle tissue with TMEM38A antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | TMEM38A antibody can be used for detection of TMEM38A by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TMEM38A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TMEM38A Antibody is supplied in PBS containing 0.02% sodium azide. |
