Application
Western blot analysis of TMEM38B in rat thymus tissue lysate with TMEM38B antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TMEM38B in mouse thymus tissue with TMEM38B antibody at 20 μg/mL.Application
Immunohistochemistry of TMEM38B in mouse thymus tissue with TMEM38B antibody at 5 μg/mL.| Product Name | TMEM38B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TMEM38B Antibody: OI14, TRICB, TRIC-B, C9orf87, D4Ertd89e, bA219P18.1, Trimeric intracellular cation channel type B, Transmembrane protein 38B |
| Product description | TMEM38B Antibody: TMEM38A and TMEM38B are two recently identified trimeric intracellular cation (TRIC) channel subtypes. TMEM38B is expressed in most mammalian tissues, while TMEM38A is preferentially expressed in excitable tissues such as striated muscle and brain. Mice deficient in both TMEM38A and TMEM38B suffer embryonic cardiac failure; the cardiac myocytes display severe dysfunction in SR Ca2+ handling, weakened Ca2+ release, and reduced K+ permeability indicating that the TRIC cation channels are likely to act as counter-ion channels that function in synchronization with Ca2+ release from intracellular stores. Mice that were lacking only TMEM38B however, die shortly after birth due to respiratory failure and have lungs exhibiting severe histological defect and ultrastructural abnormalities in their alveolar type II epithelial cells, indicating that TMEM38B are essential for perinatal lung maturation. Other experiments have shown that TMEM38A and TMEM38B can act with junctophilin proteins to support efficient ryanodine receptor-mediated Ca2+ release in muscle cells.1) Yazawa M, Ferrante C, Feng J, et al. TRIC channels are essential for Ca2+ handling in intracellular stores. Nature2007; 448:78-82. |
| Immunogen | TMEM38B antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human TMEM38B. The immunogen is located within the last 50 amino acids of TMEM38B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TMEM38B antibody can be used for detection of TMEM38B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM38B |
|---|---|
| Alternative Names | TMEM38B Antibody: OI14 TRICB TRIC-B C9orf87 D4Ertd89e bA219P18.1 Trimeric intracellular cation channel type B Transmembrane protein 38B |
Application
Western blot analysis of TMEM38B in rat thymus tissue lysate with TMEM38B antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TMEM38B in mouse thymus tissue with TMEM38B antibody at 20 μg/mL.
Application
Immunohistochemistry of TMEM38B in mouse thymus tissue with TMEM38B antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1471 - Rat Thymus Tissue Lysate |
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| Application Notes | TMEM38B antibody can be used for detection of TMEM38B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TMEM38B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TMEM38B Antibody is supplied in PBS containing 0.02% sodium azide. |
