Application
Western blot analysis of TMEM59 in human kidney tissue lysate with TMEM59 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TMEM59 in human kidney tissue with TMEM59 antibody at 20 μg/mL.Application
Immunohistochemistry of TMEM59 in human kidney tissue with TMEM59 antibody at 2.5 μg/mL.| Product Name | TMEM59 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TMEM59 Antibody: C1orf8, HSPC001, C1orf8, UNQ169/PRO195, Transmembrane protein 59, Liver membrane-bound protein |
| Product description | TMEM59 Antibody: Processing of the amyloid precursor protein (APP) by two different proteases, called alpha- and beta-secretase, is a central regulatory event in the generation of the amyloid beta peptide (Abeta), which has a key role in Alzheimer disease (AD) pathogenesis. TMEM59, a Golgi-localized protein, modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP. It inhibits APP transport and shedding.1) Schöbel S, Neumann S, Seed B, et al. Expression cloning screen for modifiers of amyloid precursor protein shedding. Int. J. Dev. Neurosci.2006; 24:141-8. |
| Immunogen | TMEM59 antibody was raised against a 16 amino acid synthetic peptide near amino terminus of human TMEM59. The immunogen is located within amino acids 80 - 130 of TMEM59. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TMEM59 antibody can be used for detection of TMEM59 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM59 |
|---|---|
| Alternative Names | TMEM59 Antibody: C1orf8 HSPC001 C1orf8 UNQ169/PRO195 Transmembrane protein 59 Liver membrane-bound protein |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
Application
Western blot analysis of TMEM59 in human kidney tissue lysate with TMEM59 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TMEM59 in human kidney tissue with TMEM59 antibody at 20 μg/mL.
Application
Immunohistochemistry of TMEM59 in human kidney tissue with TMEM59 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | TMEM59 antibody can be used for detection of TMEM59 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TMEM59 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TMEM59 Antibody is supplied in PBS containing 0.02% sodium azide. |
