Application
Western blot analysis of TMEM59L in rat heart tissue lysate with TMEM59L antibody at 1 μg/mL.Application
Immunofluorescence of TMEM59L in mouse brain tissue with TMEM59L antibody at 20 μg/mL.Application
Immunohistochemistry of TMEM59L in mouse brain tissue with TMEM59L antibody at 2.5 μg/mL.| Product Name | TMEM59L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TMEM59L Antibody: BSMAP, C19orf4, BSMAP, Transmembrane protein 59-like, Brain-specific membrane-anchored protein |
| Product description | TMEM59L Antibody: Processing of the amyloid precursor protein (APP) by two different proteases, called alpha- and beta-secretase, is a central regulatory event in the generation of the amyloid beta peptide (Abeta), which has a key role in Alzheimer disease (AD) pathogenesis. TMEM59L is a type I membrane glycoprotein that is expressed in the brain. A related protein, TMEM59, is a Golgi-localized protein that modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP and inhibits APP transport and shedding.1) Schöbel S, Neumann S, Seed B, et al. Expression cloning screen for modifiers of amyloid precursor protein shedding. Int. J. Dev. Neurosci. 2006; 24:141-8. |
| Immunogen | TMEM59L antibody was raised against a 19 amino acid synthetic peptide near carboxy terminus of human TMEM59L. The immunogen is located within amino acids 220 - 270 of TMEM59L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TMEM59L antibody can be used for detection of TMEM59L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM59L |
|---|---|
| Alternative Names | TMEM59L Antibody: BSMAP C19orf4 BSMAP Transmembrane protein 59-like Brain-specific membrane-anchored protein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (89%) |
| Tissue Specificity | Multiple isoforms of TMEM59L are known to exist. TMEM59L antibody is predicted to not cross-react TMEM59. |
Application
Western blot analysis of TMEM59L in rat heart tissue lysate with TMEM59L antibody at 1 μg/mL.
Application
Immunofluorescence of TMEM59L in mouse brain tissue with TMEM59L antibody at 20 μg/mL.
Application
Immunohistochemistry of TMEM59L in mouse brain tissue with TMEM59L antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1461 - Rat Heart Tissue Lysate |
|---|---|
| Application Notes | TMEM59L antibody can be used for detection of TMEM59L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TMEM59L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TMEM59L Antibody is supplied in PBS containing 0.02% sodium azide. |
