Application
Western blot analysis of TMEM70 in human liver tissue lysate with TMEM70 antibody at 1 μg/mL.Application
Immunofluorescence of TMEM70 in rat liver tissue with TMEM70 antibody at 20 μg/mL.Application
Immunohistochemistry of TMEM70 in rat liver tissue with TMEM70 antibody at 5 μg/mL.| Product Name | TMEM70 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TMEM70 Antibody: MC5DN2, Transmembrane protein 70, mitochondrial |
| Product description | TMEM70 Antibody: TMEM70 is a recently identified mitochondrial protein that is thought to play a role in the biogenesis of the ATP synthase in higher eukaryotes. Mutations in this gene result in early neonatal onset of hypotonia, hypertrophic cardiomyopathy, lactic acidos and 3-methylglutaconic aciduria (3-MGC-uria), and usually cause death within the first six weeks of life, although some patients survive much longer. Little is known of the role of TMEM70, but it is conserved across multicellular eukaryotic organisms. It contains a conserved DUF1301 domain and two putative transmembrane regions.1) Cizkova A, Stranecky V, Mayr JA, et al. TMEM70 mutations cause isolated ATP synthase deficiency and neonatal mitochondrial encephalocardiomyopathy. Nat. Genet.2008; 11:1288-90. |
| Immunogen | TMEM70 antibody was raised against a 14 amino acid synthetic peptide near the center of human TMEM70. The immunogen is located within amino acids 150 - 200 of TMEM70. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TMEM70 antibody can be used for detection of TMEM70 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM70 |
|---|---|
| Alternative Names | TMEM70 Antibody: MC5DN2 Transmembrane protein 70 mitochondrial |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (93%) |
Application
Western blot analysis of TMEM70 in human liver tissue lysate with TMEM70 antibody at 1 μg/mL.
Application
Immunofluorescence of TMEM70 in rat liver tissue with TMEM70 antibody at 20 μg/mL.
Application
Immunohistochemistry of TMEM70 in rat liver tissue with TMEM70 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | TMEM70 antibody can be used for detection of TMEM70 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TMEM70 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TMEM70 Antibody is supplied in PBS containing 0.02% sodium azide. |
