Application
Western blot analysis of TMEM88 in human brain tissue lysate with TMEM88 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TMEM88 in mouse brain tissue with TMEM88 antibody at 20 μg/mL.Application
Immunohistochemistry of TMEM88 in mouse brain tissue with TMEM88 antibody at 2.5 μg/mL.| Product Name | TMEM88 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TMEM88 Antibody: TMEM88A, Transmembrane protein 88 |
| Product description | TMEM88 Antibody: Transmembrane protein 88 (TMEM88) is a two-transmembrane-type protein whose C-terminal tail has been shown to bind the PDZ domain of Dishevelled (Dvl), one of the key components in Wnt signaling pathways. TMEM88 attentuated the Wnt/beta-catenin signaling induced by Wnt-1 ligand in a dose-dependent manner and knockdown of TMEM88 by RNAi increased Wnt activity, suggesting that TMEM88 plays a role in regulating Wnt signaling in a context-dependent manner.1) Lee HJ, Finklestein D, Li X, et al. Identification of transmembrane protein 88 (TMEM88) as a dishevelled-binding protein. J. Biol. Chem. 2010; 285:41549-56. |
| Immunogen | TMEM88 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TMEM88. The immunogen is located within amino acids 110 - 160 of TMEM88. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TMEM88 antibody can be used for detection of TMEM88 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM88 |
|---|---|
| Alternative Names | TMEM88 Antibody: TMEM88A Transmembrane protein 88 |
| Tissue Specificity | At least two isoforms of TMEM88 are known to exist; this antibody will recognize both isoforms. |
Application
Western blot analysis of TMEM88 in human brain tissue lysate with TMEM88 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TMEM88 in mouse brain tissue with TMEM88 antibody at 20 μg/mL.
Application
Immunohistochemistry of TMEM88 in mouse brain tissue with TMEM88 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | TMEM88 antibody can be used for detection of TMEM88 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TMEM88 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TMEM88 Antibody is supplied in PBS containing 0.02% sodium azide. |
