Application
Western blot analysis of TMP21 in mouse brain tissue lysate with TMP21 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunohistochemistry of TMP21 in human brain tissue with TMP21 antibody at 5 μg/mL.| Product Name | TMP21 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TMP21 Antibody: p23, TMP21, S31I125, Tmp-21-I, S31III125, P24(DELTA), Transmembrane emp24 domain-containing protein 10, 21 kDa transmembrane-trafficking protein |
| Product description | TMP21 Antibody: TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta; production, demonstrating that TMP21 can modulate gamma-secretase activity.1) Blunt R, Feick P, Puype M, et al. Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking. J. Biol. Chem.1996; 271:17183-9. |
| Immunogen | TMP21 antibody was raised against a 18 amino acid synthetic peptide from near the center of human TMP21. The immunogen is located within amino acids 70 - 120 of TMP21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| TMP21 antibody can be used for detection of TMP21 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMED10 |
|---|---|
| Alternative Names | TMP21 Antibody: p23 TMP21 S31I125 Tmp-21-I S31III125 P24(DELTA) Transmembrane emp24 domain-containing protein 10 21 kDa transmembrane-trafficking protein |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of TMP21 in mouse brain tissue lysate with TMP21 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunohistochemistry of TMP21 in human brain tissue with TMP21 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | TMP21 antibody can be used for detection of TMP21 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TMP21 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TMP21 Antibody is supplied in PBS containing 0.02% sodium azide. |
