Application
Western blot analysis of TMPRSS2 antibody in HepG2 cell line lysates (35ug/lane)Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TMPRSS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transmembrane protease serine 2, 3421-, Serine protease 10, Transmembrane protease serine 2 non-catalytic chain, Transmembrane protease serine 2 catalytic chain, TMPRSS2, PRSS10 |
| Product description | TMPRSS2 is a protein that belongs to the serine protease family. The protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. Its gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage.1) Gopalan,A., Cancer Res. 69 (4), 1400-1406 (2009) |
| Immunogen | This TMPRSS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the Central region of human TMPRSS2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TMPRSS2 |
|---|---|
| Alternative Names | Transmembrane protease serine 2 3421- Serine protease 10 Transmembrane protease serine 2 non-catalytic chain Transmembrane protease serine 2 catalytic chain TMPRSS2 PRSS10 |
| Molecular Weight(MW) | 54 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of TMPRSS2 antibody in HepG2 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
